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Anticlump-Cytocaf


Cytocaf

Anticlump-Cytocaf is a recombinant protein polymer designed to prevent unwanted cell aggregation in suspension cultures. It forms a gentle, physical barrier that reduces cell–cell contact, minimising aggregation while maintaining a healthy, non-disruptive environment for cell growth.

In the presence of Anticlump-Cytocaf, a wide range of cells, including mammalian cell lines and both animal and human induced pluripotent stem cells (iPSCs), show improved viability and significantly reduced aggregation. Anticlump-Cytocaf is highly stable, simple to use, and seamlessly integrates into existing culture workflows, offering broad compatibility across different media, formats, and protocols.

  • Prevents clumping in suspension cell cultures
  • Data shows transfection is not affected by Anticlump-Cytocaf
  • Enhances cell viability across multiple cell types, including iPSCs
  • Ideal for human and animal stem cell workflows
  • Easy to use and stable under a variety of culture conditions
  • Compatible with standard lab protocols

Figure 1: HEK293 cells

Cytocaf-AntiClump allows adherent cells to grow in suspension without clumping.

Wells of a non-TC coated plastic plate were seeded with HEK293 cells in media containing 10% FBS and grown for 72 hours.
Wells of a non-TC coated plastic plate were seeded with HEK293 cells in media containing 10% FBS and 0.25 mg/mL Cytocaf-AntiClump. Cells were then grown for 72 hours.
Anticlump-Cytocaf

(A) Wells of a non-TC coated plastic plate were seeded with 1.9x104 cells/mL HEK293 cells in media containing 10% FBS and grown for 72 hours. Images were taken by bright field microscopy at 10X magnification.

(B) Wells of a non-TC coated plastic plate were seeded with 1.9x104 cells/mL HEK293 cells in media containing 10% FBS and 0.25 mg/mL Cytocaf-AntiClump. Cells were then grown for 72 hours. Images were taken by bright field microscopy at 10X magnification.

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AntiClump-Cytocaf
AntiClump-Cytocaf
Since 1965

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